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Santa Cruz Biotechnology
rabbit anti epha2 antibody  Rabbit Anti Epha2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/epha2+d4a2/pmc07850503-321-19-38?v=Santa+Cruz+Biotechnology Average 96 stars, based on 1 article reviews
rabbit anti epha2 antibody - by Bioz Stars,
2026-07
96/100 stars
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Bethyl
rabbit anti epha2 Rabbit Anti Epha2, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/epha2+d4a2/pm28883622-407-41-67?v=Bethyl Average 93 stars, based on 1 article reviews
rabbit anti epha2 - by Bioz Stars,
2026-07
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Proteintech
epha2 d4a2 Epha2 D4a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/epha2+d4a2/pmc08945957-51-34-33?v=Proteintech Average 93 stars, based on 1 article reviews
epha2 d4a2 - by Bioz Stars,
2026-07
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The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors
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Image Search Results
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (A) Effects of epidermal growth factor (EGFR) siRNA or control (ctrl) siRNA on the time course of EphA2 and EGFR phosphorylation in OKF6/TERT-2 oral epithelial cells infected with C . albicans . Results are representative of 3 independent experiments. Densitometric quantification of all 3 immunoblots such as the one in (A) is shown in . (B) Proximity ligation assay showing the physical association between EphA2 and EGFR in uninfected (Ctrl) and C . albicans (Ca) infected epithelial cells after 90 min. Red spots indicate regions of EphA2-EGFR association. Scale bar 10 μm. (C) Effects of EphA2 and EGFR siRNA on the surface expression of EphA2 and EGFR by oral epithelial cells, as determined by flow cytometry. Immunoblots demonstrating the extent of siRNA knockdown in whole cell lysates are shown in . Results are representative of 3 independent experiments.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Control, Phospho-proteomics, Infection, Western Blot, Proximity Ligation Assay, Expressing, Flow Cytometry, Knockdown
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (A) Immunoblot analysis showing the effects of the indicated C . albicans strains on the phosphorylation of EphA2 and EGFR after 30 and 90 min post-infection (p. i.). Results are representative a 3 independent experiments. Densitometric quantification of all 3 immunoblots such as the one in (A) is shown in . (B) Extent of oral epithelial cell damage caused by the indicated strains after 8 h of infection. Box whisker plots show median, interquartile range, and range of 3 independent experiments, each performed in triplicate. Statistical significance was determined by the Kruskal-Wallis test corrected for multiple comparisons. ***, p < 0.001; ****, p < 0.0001. Confocal microscopic images of oral epithelial cells after 90-min infection with candidalysin-V5 expressing strains of C . albicans . Arrows indicate endocytosed C . albicans hyphae with detectable candidalysin-V5. Boxes indicate the areas magnified in the insets. Scale bar: 40 μm.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Western Blot, Phospho-proteomics, Infection, Whisker Assay, Expressing
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (A) Immunoblot showing the phosphorylation of EGFR on tyrosines 845, 992, and 1045 in response to 90-min infection of oral epithelial cell with the indicated strains of C . albicans . Results are representative 3 independent experiments. Densitometric quantification of all 3 immunoblots such as the one in (A) is shown in . (B) Effects of siRNA knockdown of EGFR and EphA2 on the production of the indicated cytokines and chemokines in response to 8 h of infection with C . albicans (Ca). (C) Effects of 8 h infection of oral epithelial cells with the indicated strains of C . albicans on secretion of the indicated cytokines and chemokines. Data were analyzed using the Kruskal-Wallis test corrected for multiple comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001; **** p , < 0.0001.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Western Blot, Phospho-proteomics, Infection, Knockdown
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (A and B) Immunoblots showing effects of 1 μg/ml ephrin A1-Fc (EFNA1-Fc) or yeast-phase C . albicans SC5314 (Ca) on the phosphorylation of ephrin type-A receptor 2 (EphA2) in OKF6/TERT-2 oral epithelial cells after stimulation for 15 min (A) and 60 min (B). (C and D) Effects of EFNA1-Fc and 100 ng/ml epidermal growth factor (EGF) on the phosphorylation and total cell levels of EphA2 (C) and EGFR (D). (E and F) Effects of 70 μM candidalysin (CDLY) on the phosphorylation and total cell levels of EphA2 (E) and EGFR (F) Results in A-F are representative of 3 independent experiments. Densitometric quantification of all 3 immunoblots is shown in . (G) Effects of 8-h incubation with EFNA1-Fc, EGF, zymosan (zym), and candidalysin on the epithelial cell production of the indicated cytokines and chemokines.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Western Blot, Phospho-proteomics, Incubation
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (A) Adherence (cell-association) and endocytosis of wild-type C . albicans strain SC5314 by OKF6/TERT-2 and TR146 oral epithelial cell lines. (Orgs/10 HPF, organisms per 10 high-power fields) (B) Comparison of the effects of gefitinib (GEF) versus diluent (ctrl) on C . albicans adherence to and endocytosis by OKF6-TERT-2 and TR146 cells. (C) Time course of C . albicans -induced damage to OKF6-TERT-2 and TR146 cells. (D) Extent of damage to OKF6/TERT-2 and TR146 cells caused by C . albicans and Triton X-100 (TX-100) after 24 h incubation. (E) Comparison of the extent of phosphorylation on the indicated tyrosine residues of EGFR in unstimulated OKF6/TERT-2 and TR146 cells. (F) Phosphorylation of EphA2 and EGFR in TR146 cells after 30 and 60 min post-infection (p.i.) with C . albicans . Results in (E and F) are representative immunoblots from 3 independent experiments. Densitometric quantification of all 3 immunoblots is shown in . (G) Comparison of the basal release of the indicated inflammatory mediators by uninfected OKF6-TERT-2 and TR146 cells. Results are the ratio of TR146 cells to OKF6/TERT2 cells. (H) Effects of gefitinib on C . albicans -induced production of the indicated pro-inflammatory mediators by TR146 cells. Graphs show the results of 3 experiments, each performed in triplicate (A-D) or duplicate (G and H). The data in (A, B, and D) were analyzed with the Mann-Whitney test, and the data in (C, G, and H) were analyzed by the Kruskal-Wallis test corrected for multiple comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001; **** p , < 0.0001.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Comparison, Incubation, Phospho-proteomics, Infection, Western Blot, MANN-WHITNEY
Journal: PLoS Pathogens
Article Title: Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors
doi: 10.1371/journal.ppat.1009221
Figure Lengend Snippet: (Left panel) Exposed β-glucan on the surface of a C . albicans cell interacts with and activates EphA2, priming the epithelial cell to respond to C . albicans . (Right panel) When the C . albicans cell germinates, it expresses Als3 and secretes candidalysin, which activate EGFR and prevent the EphA2-EGFR complex from being degraded. Als3 induces the epithelial cells to endocytose the hypha, leading to the formation of an endocytic vacuole in which the secreted candidalysin can accumulate. The higher concentration of candidalysin damages the epithelial cell and stimulates it to secrete pro-inflammatory cytokines and chemokines by both EGFR-dependent and EGFR-independent signaling pathways.
Article Snippet: After the cells were permeablized with 0.1% Triton X-100 in PBS for 15 min, they were incubated with a
Techniques: Concentration Assay, Protein-Protein interactions